In the “Pipetting” menu, you can set several options according to the needs of your application. These pipetting parameters are organized in four sections: Configuration, Advanced, Automation and Guidelines.
Besides single pipetting actions where you can set a single volume for a single transfer, or a single volume for multiple transfers in one go, you can also design multi-volume pipetting actions. During a multi-volume pipetting action, you can choose to transfer arbitrary amounts of liquid from/to the several sources/destinations of the labware you selected. You may find this option under the “Configuration” section of the “Pipetting” menu.
To design a multi-volume pipetting step, first design a single pipetting action (see the article “Single pipetting action”) where reagent is transferred from a single source to multiple destinations. You can either choose a labware with multiple locations as a destination or several individual pieces of labware. In this example, a 96 well microplate is used with dispensing locations the first column of the plate (see the article “Selecting sources and/or destinations on a labware with multiple positions manually”).
As with the case for a single pipetting action, the “Pipetting” menu will allow you to change the pipetting parameters according to your preferences.
Under "Configuration” you have the option to activate the “Multi-volume mode”, as shown in the figure below:
Choosing this option expands the “Configuration” section, allowing you to update/overwrite your source locations, destination locations, as well as the amounts of reagent that will be transferred per destination (and per source location if applicable). This can be done manually, or by pasting from an external source (see the articles “Define volumes by importing information from an external source”, “Selection of labware locations by importing information from an external source” and “Importing reagents at start (stock solutions) from an external source”).
The “Configuration” section once expanded contains:
- The destinations listed in the table according your selection.
- You can change the source and destination locations in the table by pasting from an external source (ex. Excel, Notepad etc).
- Finally, you can define the multiple volumes either by hand, or by pasting the data from an external source (ex. Excel, Notepad etc).