The liquids that are present in different labware at the start of the experiment, before any (pipetting) actions are performed are called “Reagents”. Reagents are tracked during the protocol and therefore, it is always clear which labware contain which reagents at each protocol step.
You can add reagents to any labware present on the virtual bench. At the moment you have to do this manually per labware (see the article “Fill a labware with a reagent”). In future updates of the software, importing the data from an external source will be made possible as well. This functionality is covered by the articles “Selection of labware locations by importing information from an external source”, “Define volumes by importing information from an external source” and "Importing reagents at start (stock solutions) from an external source"
Reagents have a name, a volume and a concentration. During the protocol, the location of each reagent is tracked by the software and during mixing with other reagents, the concentrations are calculated automatically by the software. The minimum required amount of reagent is automatically calculated by the software, depending on the needs during the protocol. You can also set the amount manually to a fixed value. OneLab gives a warning if there is not enough liquid present to be able to execute the protocol.