One of the most frequent actions you will have to implement in OneLab is a single pipetting step. This action can represent one of the following case scenarios:
- You want to transfer a certain amount of liquid from one single labware to another single labware. An example is a transfer of 1 mL from a large 50 mL tube to a small 1.5 mL microtube.
- You want to transfer a certain amount of liquid from one single labware to multiple destinations. This can be several separate labware, or a labware that has a larger number of destinations, for example a microplate with an X number of wells. An example is a transfer of 100 uL from a 50 mL tube to each of the wells of the first column of a microplate.
- You want to transfer a certain amount of liquid from multiple source locations to multiple destinations. This can be from X wells to X wells on the same microplate, or from Y wells on one microplate to Y wells on another microplate. In this case, the number of source - and destination locations should be the same. An example is to transfer 50 uL from wells A4-H4 of microplate #1 to the wells A5-H5 of microplate #2.
- You want to pre-wet your tip by aspirating and dispensing in the same labware (source and destination labware are the same). Normally for such action, the mixing option at source or destination is enabled. For more details see the articles “Advanced: pipetting”, “Advanced: handling liquid viscosity”, “Advanced: tip position”, “Advanced: tip choice" and “Advanced: mixing”.
The four possibilities are shown in the following figures:
Note: Labware that contains liquid is colored, compared to empty labware that is blank. When liquid is transferred from a source to a destination and the destination was previously empty, it gets a color during the process.
You may implement each of the above actions as one single pipetting step in your OneLab protocol via one of the following ways:
- Drag from one labware (source) to another (destination). The software will show an arrow pointing away from the source. The end of the arrow indicates where you will dispense the liquid and becomes final when the dragging movement is completed at the desired destination. The “Select sources” and/or “Select destinations” menus will popup automatically when handling a labware with multiple locations. For more information, see the article “Selecting sources and/or destinations on a labware with multiple locations manually”.
Dragging implements directly a single pipetting action from a source – to a destination labware. Intermediate menus will popup automatically when required, i.e. the “Pipetting”, “Select sources” and “Select destinations” menus. In this example, each of the wells in the first column of the microplate are filled with 100 uL of liquid coming from the 15mL Falcon tube.
The last step that you need to take to finalize the implementation of a single pipetting action, is to fill out the desired pipetting parameters (see the article “Pipetting menu" for more information). It contains on the left the Sources and Destinations for this specific protocol step and four parts that you need to go through: Configuration, Advanced, Automation and Guidelines.
2. Alternatively, click on the “Actions menu” button in the “Protocol Menu Bar” on the left of your screen. In the pop-up “Actions” menu you can now click on the “Pipetting” button. In the next menu called “X. Pipetting”, you have to define the source labware and the destination labware (the X refers to the protocol step you are presently editing). If the source - and/or destination labware has multiple wells (microplate), additional menus will open automatically. These are logically called “Select sources” and “Select destinations”. Once confirmed, you need to input the required information about the liquid transfer and pipetting parameters. The steps you need to follow are shown here as a series of screenshots:
Add the required labware to the virtual workbench ( in this example: a 14mL Falcon tube and a 96-well microplate), see the article “Adding labware to the virtual bench” for more information.
Step A: Click on the “Actions” menu button in the “Protocol Menu Bar” on the left of your screen. The “Actions” menu will popup. Then, click on the “Pipetting” option.
Step B: In the “Pipetting menu” define the sources and destinations by dragging the labware of your choice to the respective fields on the right of your screen.
Step C: The intermediate status of this example is shown here: the 15mL Falcon tube was dragged to the “Sources” field and the 96-well microplate to the “Destinations” field. As soon as a labware is dragged to either the “Sources” or “Destinations”field, automatically a new menu appears if the labware has multiple locations(wells). In this example, the “Select destinations” menu will thus open. How to select the wells on a microplate is explained in the article “Selecting sources and/or destinations on a labware with multiple locations manually”. As soon as the “Select destinations”menu is closed, you will get back to the “Pipetting menu”, even if you did not select any wells
Note: In this example, the “Destinations” field has the green text “Select wells” present and it is indicated that no wells were selected in the “Select destinations” menu. Clicking on the “Select wells” gives you the possibility to go back to this menu and edit which wells should be used to dispense liquid.
Step D: As soon as valid sources and destinations are chosen, the final status of the “Pipetting” menu is reached and the “Continue” button on the top right of your screen becomes enabled.
Important note: In case no reagent is defined in your source labware, OneLab will warn you during the next implementation step of this action that an inconsistency occurred. To correct this error, add a reagent to the source labware as mentioned in the “Fill a labware with a reagent” article. Finally, you may find a detailed description about all options you can select during a pipetting action in the article “Pipetting menu".