You design your protocols to act as a guideline for how to execute an experiment that involves different pipetting steps. With OneLab, these steps can be executed as follows: 

  1. Manual pipetting: You are guided through each pipetting step of the protocol by OneLab and you indicate when each step has been executed, by clicking an on-screen button.
  2. Semi-automatic pipetting with Pipette+: Using Andrew Alliance connected electronic pipettes you are guided through each pipetting step of the protocol while OneLab displays instructions for the current step of the protocol and automatically advances through subsequent steps as you pipette until the end of the protocol has been reached.
  3. Automatic pipetting with Andrew+: All you needto do is set up the experiment, following the guidance provided by OneLab,then the Andrew+ robot will carry out each step of the protocolautomatically.

You can only execute semi-automatically or automatically when Pipette+ or Andrew+ are available and paired up with your OneLab Lab. See the articles “What is Pipette+?” and “What is Andrew+?” for more information. You can check if you have connected devices in your lab by going to the Devices tab. When no devices are available yet, there are two links to the Andrew Alliance website, one for Pipette+ and one for Andrew+. The Devices tab in such case looks as follows:

Since no devices are available, you can only execute the protocols you designed manually. So this is the only option given in the “Execution of ‘protocol name’” menu, shown in the following figure:

You can start the execution of your protocol in different ways:

  1. By clicking on the green “Execute” button (highlighted by the red ellipse in the figure below) on the Projects tab (1), in the list of protocols in one of your project folders (2).
  2. By clicking on the arrow button in the menu of the protocol designer (second figure below).

In case devices are available, they are listed as shown in the image below along with their status (“ready”, ”busy” and “offline”):

By starting the execution of a protocol, you can now choose from three options: manually, semi-automated with Pipette+ and fully automated with Andrew+. Each of these options has a dropdown list on the left side, indicated by the red arrows in the figure. In the dropdown list, all the available devices of a certain kind are listed, to choose the one you want.

Execution times can be compared, and the most optimal mode of execution can be chosen. Note that the duration of the fully automated execution with the Andrew+ robot is expected to be the same as the manual execution duration, but the hands-on time is reduced to zero.

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