After having added your labware to the virtual bench (see the “Adding labware to the virtual bench” article), you can easily fill it with reagents.
There are two ways to do so, described below and shown in the following 3 figures:
Click on the labware you would like to fill.A small black contextual menu bar will appear close to the selected labware. Depending on the type of labware one of the following options will appear:
a. In case of a single-location labware, there is a label showing an icon of a droplet of liquid with a plus logo,accompanied with the word “Add”. When you click the aforementioned button the “Fill ‘name of labware’ with reagent” menu will appear.
b. In case of a multi-location labware, the label shows “Inspect wells” instead. When you click here, you will see an enlarged version of the labware, where you can select the locations of interest (see the article“Selecting sources and/or destinations on a labwarewith multiple locations manually"). After selecting the locations, you can add a reagent (see step 2b).
Alternatively select your desired labware on the virtual bench and go to the Labware Info tab on the top right corner. Also here the menu is different according to which type of labware you selected beforehand.
a. In case you selected a single-location labware, a green button is available, labelled “Add reagent at start”. When you click on it, the “Fill ‘name of labware’ with reagent” menu will appear.
b. In case of a multi-location labware, you first have to click the button “Select wells” (see step 1b). After the selection, again like for 2a, the “Add reagent at start” button is available.
Please note that the menu title is specific to the labware you selected on the virtual bench. To remind you which labware you are filling, the menu title has a comprehensive name. In the following example, the 50mL Falcon conical centrifuge tube was selected to be filled with reagent:
The “Fill ‘name of labware’ with reagent” menu that pops up automatically, gives you several options:
You can scroll through all the predefined reagents and select the one you desire.
You can search for a reagent by using the search field on the top right side.
You can apply filters (settings on the left) according to your preference.
You can create a new reagent with the properties you want that will be available for future use as well. See the article “Create a new reagent”.
You can close the menu by clicking on the "X" on the top left.
Once you have selected a reagent for your labware, the “Fill ‘name of labware’ with reagent” menu is automatically closed and you are redirected to the virtual bench. On the Labware Info tab, you can set a fixed volume, or leave the default “AUTO calculation” checkbox checked, such that the OneLab software automatically takes into consideration all implemented pipetting steps and sets the precise value for the overall volume that is required to perform all actions during the entire protocol. This is indicated by the red arrows in the following figure.
On the virtual bench, the small black contextual menu bar, attached to the labware you selected, now does not have an “Add” button anymore, but rather an “x reagent” button (in this case 1 reagent). By clicking this button you will be able to inspect which reagent is present in the labware, what the concentration is (if applicable) and how much volume of it is present/required. Especially when dilutions or normalization are performed, the concentration is obviously a very important parameter. For multi-location labware, the button label stays as it was: “Inspect wells”.
Besides filling a labware with the reagent of your choice, in case of a mistake, it is of course possible to remove the reagent or to replace it with another reagent. In the figure above, this is indicated by the red ellipse. The button next to it, removes the reagent from the labware.
An alternative way to replace a reagent, is to first click on the reagent name in the black contextual menu bar and then click on the pencil icon in the pop-up menu to enter the “Fill ‘name of labware’ with reagent” menu. The fact that the labware now contains a reagent is reflected in the representation of the labware on the virtual bench: it is given an arbitrary color, instead of being black/white.
As indicated in the figure above, the total volume at the start of the experiment is 1.2 mL, because the “AUTO calculation” checkbox was unchecked. In case of the “AUTO calculation” checked, the value of 0 uL at start will be indicated here, even if you defined a reagent.
Filling a labware with multiple locations
For labware with multiple locations, like microplates with many wells or matrices with many tubes, the “reagent at start” can only be set manually in this first release of OneLab. Locations (wells) can be imported from an external file (see the article: “Selection of labware locations by importing information from an external source”). When the desired locations are selected (either manually or imported), you can add the SAME reagent to ALL of these locations simultaneously, where ALL locations will have the SAME volume as well (either “Auto” or a fixed value). If you prefer to have different reagents (or different volumes) at the various locations of the same labware, you are required to manually define this information for each location individually.
To import “reagents at start” by supplying copied information from an external file with single location or column-wise information: location (well), reagent name, concentration (optional), units of the concentration (optional), and the volume (optional), see the article “Importing reagents at start (stock solutions) from an external source”.