In the “Pipetting” menu, you can set several options according to the needs of your application. These pipetting parameters are organized in four sections: Configuration, Advanced, Automation and Guidelines.
Under the “Advanced/Tip position” section in the “Pipetting” menu, you can set the position where the pipette tip should be immersed, suiting your application best while aspirating at the source. You can choose between “With respect to liquid” and “With respect to bottom”.
In some cases, you might be required to aspirate from the bottom of your labware, since the reagent you want to transfer collects on the bottom (settles), while in other cases following the liquid surface might be a better option.
Similarly, during the dispensing at the destination, you can choose between these options to set the height of the tip:
With respect to liquid: With this option the robot will track the liquid height as it pipettes
With respect to bottom: select this option only when the tip will not get submerged
On-the-fly: should be enabled when the tip should remain on top of the destination labware without being in contact with the reagent. This option is recommended when you would like to avoid bubble formation when certain reagents are used and contamination (of the source) if the same tip is reused for several transfers. Beware that this height above the consumables may result in splatter effects of certain liquids with droplets or mist ending up outside the target destination, potentially causing contamination of samples in other labware.