Introduction

Correct liquid aspiration and dispensing speed are fundamental to reproducibility and accuracy during liquid handling.
For additional information, check out Andrew Alliance 💧 liquid handling.

In all main pipetting modes, speed can be adjusted separately for aspiration

and dispensing.

The tables below report for each pipette type the required time in seconds to aspirate or dispense distilled water when the maximum volume is set.


Single-channel pipettes (speed in seconds)

Pipette

➡️

Speed
⬇️

10 µL

120 µL

300 µL

1,000 µL

5,000 µL

10,000 µL

1

2.5

6.0

7.7

10.1

10.2

10.2

2

1.8

4.2

5.3

7.4

7.4

7.4

3

1.3

2.9

3.7

5.4

5.4

5.4

4

1.0

2.1

2.7

3.8

3.8

3.8

5

0.8

1.5

1.9

2.8

2.7

2.9

6

0.6

1.1

1.4

1.9

1.8

2.2

7

0.5

0.9

1.1

1.2

1.1

1.7

8

0.4

0.7

0.9

0.8

0.8

1.3

9

0.3

0.6

0.8

0.6

0.6

0.9

Multi-channel pipettes (speed in seconds)

Pipette

➡️

Speed

⬇️

10 µL

120 µL

300 µL

1,200 µL

1

2.5

6.1

5.4

5.1

2

1.8

4.4

3.9

4.4

3

1.3

3.3

2.9

3.3

4

1.0

2.4

2.1

2.5

5

0.8

1.8

1.6

1.9

6

0.6

1.4

1.2

1.4

7

0.5

1.1

1.0

1.1

8

0.4

0.9

0.8

0.9

9

0.3

0.7

0.7

0.7


Conversion of liquid handling speed in OneLab

In OneLab, it is possible to set three different types of aspiration and dispensing speeds based on the viscosity of the liquid to be handled.

The table below reports the speed values set for each pipette type based on the information present in OneLab protocols.

Speed in OneLab

➡️

Pipette

⬇️

Slow

Normal

Fast

10 µL

(1-ch, 8-ch, 12-ch)

1

7

9

120 µL

(1-ch, 8-ch, 12-ch)

1

7

9

300 µL

(1-ch, 8-ch, 12-ch)

1

7

9

1,000 µL

(1-ch)

1

7

9

1,200 µL

(8-ch, 12-ch)

1

7

9

5,000 µL

(1-ch)

1

5

7

10,000 µL

(1-ch)

1

5

7

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